Study of Immunoassay Methods for Recombinant Human Erythropoietin(rhEPO)Using Competitive ELISA

Two different immunoassay methods, competitive indirect enzyme-linked immuno- sorbent assay (CI-ELISA) and amplificative competitive indirect ELISA (ACI-ELISA) using biotin-avidin complex system were studied to detect rhEPO.The linear ranges were 50-20000 ng/mL and 10-50000 ng/mL for CI-ELISA and ACI-ELISA, respectively.The low detection limits of CI-ELISA and ACI-ELISA were 62.8 ng/mL and 8.5 ng/mL, respectively.

作 者： Jin YAN Jie Bo MI Wen Bao CHANG   作者單位： The Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871  刊 名： 中國化學(xué)快報(英文版)  ISTIC SCI 英文刊名： CHINESE CHEMICAL LETTERS  年，卷(期)： 2004 15(8)  分類號：   關(guān)鍵詞： Recombinant human erythropoietin (rhEPO)   polyclonal antibody (pAb)   competitive indirect ELISA (CI-ELISA)   amplificative competitive indirect ELISA (ACI-ELISA)  

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