Parentage Analysis of Dongfang No.2, a Hybrid of a Female Gametophyte Clone of Laminariajaponica (Laminariales, Phaeophy

The cultivation of the first filial generation of monoploid gametophyte clones of different Laminaria species (hybrid Laminaria) is an effective way of utilizing heterozygous vigor (heterosis). A female gametophyte clone of L. japonica and a male gametophyte clone of L. longissima were hybridized, generating Dongfang No.2 hybrid Laminaria. The parentage of this hybrid Laminaria was determined using AFLP of total DNA, SNP of the ITS region of ribosomal RNA transcription unit and microsatellite DNA variation at two loci. In addition to 167 AFLP bands shared by Dongfang No.2, L. japonica and L. longissima, Dongfang No.2 hybrid Laminaria shared another 70 and 55 bands with L. japonica and L. longissima, respectively, which were obviously more than 11 bands shared by L. japonica and L. longissima. Dongfang No.2 held both 'T' and 'C' at position 847 of the ITS region, while 'T' at this position was specific for L. japonica and 'C' for L. longissima, respectively. Dongfang No.2 also held the mierosatellite DNA alleles of two parents together at two microsatellite DNA marker loci. These observations dearly proved that Dongfang No.2 is a true hybrid of L. japonica and L. longissiuma. Unfortunately, the origin of the chloroplast of Dongfang No.2 was not determined based on the variation of RuBisCo spacer. More sequence variants of both ITS region and RuBisCo spacer were identified in Dongfang No.2 and most of them did not exist in either L. japonica or L. longissima. The unexpected variants may be due to the mutation of ga-metophyte clones occurring during their vegetative amplification.

作 者： SHI Yuanyuan YANG Guanpin LIAO Meijie LI Xiaojie CONG Yizhou QU Shancun WANG Tongyong   作者單位： SHI Yuanyuan,YANG Guanpin,LIAO Meijie(College of Marine Life Sciences, Ocean University of China, Qingdao 266003, P. R. China)

LI Xiaojie(College of Marine Life Sciences, Ocean University of China, Qingdao 266003, P. R. China;Shandong Oriental Ocean Sci-Tec Co., Ltd., Yantai 264003, P. R. China)

CONG Yizhou,QU Shancun(Shandong Oriental Ocean Sci-Tec Co., Ltd., Yantai 264003, P. R. China)

WANG Tongyong(Wendeng Fishery Technique Popularization Station of Shandong Province, Wendeng 264400, P. R. China) 

刊 名： 中國海洋大學(xué)學(xué)報(bào)（英文版）  ISTIC 英文刊名： JOURNAL OF OCEAN UNIVERSITY OF CHINA  年，卷(期)： 2008 7(2)  分類號(hào)： P7  關(guān)鍵詞： hybrid Laminaria   gametophyte done   heterosis,Laminariajaponica   Laminaria longissima  

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