An Improved Method for Karyotype Analyses of Marine Algae

Modified carbol fuchsin staining method was successfully introduced into the karyotype analyses of marine algae, in-cluding Porphyra, Undaria pinnatifida and Laminaria japonica. Haploid chromosomes were numbered clearly in the vegetative, spermatangial and conchosporangial cells of P. haitanensis and P. yezoensis. Diploid chromosomes were observed and numbered in immature conchosporangial cells of P. haitanensis and P. yezoensis. Pit-connections of Porphyra were also clearly demonstrated. Prophase chromosomes of conchocelis cells were also clearly stained with modified carbol fuchsin. One molar per liter hydrochloric hydrolysis at 60℃ for 7-8min is necessary for getting transparent cytoplasm for conchosporangial karyotype analysis of Porphyra. Staining effects of the three methods using iron alum acetocarmine, aceto-iron-haematoxylin-chloral hydrate and modified carbol fuchsin were compared on the vegetative, sperrnatangial and conchosporangial cells of Porphyra and the gametophytes of U. pinnati-fida and L. japonica. Among the three methods, the modified carbol fuchsin method gave the best result of deep staining and good contrast between nucleus and cytoplasm.

作 者： WANG Juan DAI Jixun   作者單位： WANG Juan(Department of Life and Sciences, Heze College, Heze 274015, P. R. China)

DAI Jixun(College of Marine Life Sciences, Ocean University of China, Qingdao 266003, P. R. China) 

刊 名： 中國海洋大學(xué)學(xué)報(bào)（英文版）  ISTIC 英文刊名： JOURNAL OF OCEAN UNIVERSITY OF CHINA  年，卷(期)： 2008 7(2)  分類號： P7  關(guān)鍵詞： alga   chromosome   carbol fuchsin   staining  

【An Improved Method for Karyotype Ana】相關(guān)文章：

Study on slope reliability method Response Surface Method based on Morgenstern-Price Method04-28

Improved Preparation of Tyramine by Curtius Rearrangement04-28

ESTIMATION OF CHAIN REACTION BANKRUPTCY STRUCTURE BY CHANCE DISCOVERY METHOD- WITH TIME ORDER METHOD AND DIRECTED KEYGRA04-30

A method to obtain pulse contrast on a single shot04-28

A Method to Determine the Mean Temperature at the Planet Surface04-29

An asymptotic analysis method for the linearly shell theory04-28

Adjoint method for the optimum planning of industrial pollutant sources04-26

Knowledge Fusion Design Method: Satellite Module Layout04-26

Method of Improving the Navigation Accuracy of SINS by Continuous Rotation04-28

An operational modal analysis method in frequency and spatial domain04-29