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id="y2qj87rulgy2" class="source"><a target="_blank" href="http://www.stanzs.com/lunwenzhaiyao/shengwuyixuelunwen/" title="生物醫(yī)學(xué)論文">生物醫(yī)學(xué)論文</a></span> <span id="y2qj87rulgy2" class="source"><a target="_blank" href="http://www.stanzs.com/usr/login.html" title="我要投稿">我要投稿</a></span> </div> <script type="text/javascript">s("content_top");</script> <div id="y2qj87rulgy2" class="content"> <div id="y2qj87rulgy2" class="excellent_articles_box"> <ul> <li id="y2qj87rulgy2" class="excellent_articles_title">相關(guān)推薦</li> </ul> </div> <p>納豆激酶基因?qū)敕训难芯?/p><p>將來(lái)源于枯草芽孢桿菌的納豆激酶(nattokinase, NK)基因轉(zhuǎn)化到人類可以直接食用的,又非常喜愛(ài)的蔬菜-番茄中,得到在轉(zhuǎn)錄水平表達(dá)的轉(zhuǎn)基因植株.通過(guò)根癌農(nóng)桿菌EHA105介導(dǎo),轉(zhuǎn)化番茄的下胚軸,誘導(dǎo)愈傷組織形成及分化成幼苗,進(jìn)而再生出完整植株.提取再生植株基因組DNA,通過(guò)PCR擴(kuò)增檢測(cè),結(jié)果表明納豆激酶基因已經(jīng)成功整合到番茄植物基因組中.RT-PCR實(shí)驗(yàn)結(jié)果證明了納豆激酶基因在轉(zhuǎn)錄水平上有表達(dá).</p><p style="text-align: center;"><img loading="lazy" src="https://p.9136.com/00/l/d1d0bebf2_5ee436fbb832f.jpg" alt="納豆激酶基因?qū)敕训难芯?/></p> 作者：袁琳劉紅海王吟李曉翔楊艷燕 YUAN Lin LIU Hong-hai WANG Yin LI Xiao-xiang YANG Yan-yan 作者單位：湖北大學(xué),生命科學(xué)學(xué)院,湖北,武漢,430062 刊名：湖北大學(xué)學(xué)報(bào)（自然科學(xué)版） ISTIC PKU 英文刊名： JOURNAL OF HUBEI UNIVERSITY（NATURAL SCIENCE）年，卷(期)： 2006 28(2) 分類號(hào)： Q786 Q555+.7 關(guān)鍵詞：納豆激酶基因番茄遺傳轉(zhuǎn)化根癌農(nóng)桿菌 <script>s("content_relate");</script><p>【納豆激酶基因?qū)敕训难芯俊肯嚓P(guān)文章：</p><p><a target="_blank" href="http://www.stanzs.com/lunwenzhaiyao/shengwuyixuelunwen/20101201101315_497154.html" title="枯草桿菌納豆激酶基因在大腸桿菌中的優(yōu)化高效表達(dá)">枯草桿菌納豆激酶基因在大腸桿菌中的優(yōu)化高效表達(dá)</a><span>04-26</span></p><p><a target="_blank" href="http://www.stanzs.com/lunwenzhaiyao/shengwuyixuelunwen/20101116143159_489735.html" title="超臨界CO2流體技術(shù)制備納豆激酶腸溶微丸的初探">超臨界CO2流體技術(shù)制備納豆激酶腸溶微丸的初探</a><span>04-27</span></p><p><a target="_blank" href="http://www.stanzs.com/lunwenzhaiyao/shengwuyixuelunwen/20100702215412_173346.html" 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